At a later press conference, the police chief announced they had arrested suspect Everett Glenn Miller for premeditated first-degree murder.
Officer Matthew Baxter succumbed to his injuries, and Sgt. Sam Howard, a 10-year veteran, is in grave critical condition, according to the Kissimmee police Chief Jeffrey O'Dell.
In all, six law enforcement officers were shot Friday night in American cities: two Kissimmee police officers, two Jacksonville County Sheriff's Office deputies and two Pennsylvania State Police officers.
A police shooting was also reported Friday night in Fairchance, Pa., where two State Police troopers were shot in their auto in a supermarket shopping center, according to ABC News and WXPI-TV in Pittsburgh.
Jacksonville Sheriff Mike Williams said Saturday that officers Michael Fox and Kevin Jarrell are in stable condition following Friday night's confrontation with an armed Derrick Brabham, who was killed by the officers. Howard, who arrived as a backup, became involved in the fight when gunshots were fired and struck both officers.
Investigators are still working to understand the details of what happened in the moments before the shooting, officials said.
The Kissimmee Police Department said Sgt Sam Howard died on Saturday from his injuries. Baxter was killed. Howard was gravely wounded.
The officers - who were wearing body armor under their uniforms - apparently did not have a chance to return fire.
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Flags were lowered Saturday and a memorial was growing outside the Police Department in honor of the officers.
Miller is the only one charged of three people the police questioned, the chief said. "In Florida, justice is swiftly served against anyone who attacks our law enforcement officers".
There are no county or jurisdiction lines when it comes to our Law Enforcement Brotherhood. An early investigation found that Miller had also threatened police officers online, O'Dell said.
A unit of four to five Jacksonville police officers responded to an attempted suicide call late Friday evening.
He added, "And we're doing our part to try to keep them safe, and then we have this tonight".
Both were stable and alert, Bondarenka said. "As they were approaching the house, they knew they were staring danger in the face, and yet they moved forward to meet their commitment they held and that they swore to take for this community", Bruno said.
And two Pennsylvania state troopers were shot Friday night, the agency said.